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Enviroscan Ukrainian Institute of Speleology and Karstology

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Speleology in Kazakhstan

Shakalov on 04 Jul, 2018
Hello everyone!   I pleased to invite you to the official site of Central Asian Karstic-Speleological commission ("Kaspeko")   There, we regularly publish reports about our expeditions, articles and reports on speleotopics, lecture course for instructors, photos etc. ...

New publications on hypogene speleogenesis

Klimchouk on 26 Mar, 2012
Dear Colleagues, This is to draw your attention to several recent publications added to KarstBase, relevant to hypogenic karst/speleogenesis: Corrosion of limestone tablets in sulfidic ground-water: measurements and speleogenetic implications Galdenzi,

The deepest terrestrial animal

Klimchouk on 23 Feb, 2012
A recent publication of Spanish researchers describes the biology of Krubera Cave, including the deepest terrestrial animal ever found: Jordana, Rafael; Baquero, Enrique; Reboleira, Sofía and Sendra, Alberto. ...

Caves - landscapes without light

akop on 05 Feb, 2012
Exhibition dedicated to caves is taking place in the Vienna Natural History Museum   The exhibition at the Natural History Museum presents the surprising variety of caves and cave formations such as stalactites and various crystals. ...

Did you know?

That water logged is water saturated [16].?

Checkout all 2699 terms in the KarstBase Glossary of Karst and Cave Terms

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KarstBase a bibliography database in karst and cave science.

Featured articles from Cave & Karst Science Journals
Chemistry and Karst, White, William B.
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Featured articles from other Geoscience Journals
Karst environment, Culver D.C.
Mushroom Speleothems: Stromatolites That Formed in the Absence of Phototrophs, Bontognali, Tomaso R.R.; D’Angeli Ilenia M.; Tisato, Nicola; Vasconcelos, Crisogono; Bernasconi, Stefano M.; Gonzales, Esteban R. G.; De Waele, Jo
Calculating flux to predict future cave radon concentrations, Rowberry, Matt; Marti, Xavi; Frontera, Carlos; Van De Wiel, Marco; Briestensky, Milos
Microbial mediation of complex subterranean mineral structures, Tirato, Nicola; Torriano, Stefano F.F;, Monteux, Sylvain; Sauro, Francesco; De Waele, Jo; Lavagna, Maria Luisa; D’Angeli, Ilenia Maria; Chailloux, Daniel; Renda, Michel; Eglinton, Timothy I.; Bontognali, Tomaso Renzo Rezio
Evidence of a plate-wide tectonic pressure pulse provided by extensometric monitoring in the Balkan Mountains (Bulgaria), Briestensky, Milos; Rowberry, Matt; Stemberk, Josef; Stefanov, Petar; Vozar, Jozef; Sebela, Stanka; Petro, Lubomir; Bella, Pavel; Gaal, Ludovit; Ormukov, Cholponbek;
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Your search for units (Keyword) returned 164 results for the whole karstbase:
Showing 16 to 30 of 164
Synsedimentary collapse of portions of the lower Blomidon Formation (Late Triassic), Fundy rift basin, Nova Scotia, 1995,
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Ackermann Rv, Schlische Pw, Olsen Pe,
A chaotic mudstone unit within the lower Blomidon Formation (Late Triassic) has been traced for 35 km in the Mesozoic Fundy rift basin of Nova Scotia. This unit is characterized by highly disrupted bedding that is commonly cut by small (<0.5 m) domino-style synsedimentary normal faults, downward movement of material, geopetal structures, variable thickness, and an irregular, partially faulted contact with the overlying unit. The chaotic unit is locally overlain by a fluvial sandstone, which is overlain conformably by mudstone. Although the thickness of the sandstone is highly variable, the overlying mudstone unit exhibits only gentle regional dip. The sandstone unit exhibits numerous soft-sediment deformation features, including dewatering structures, convoluted bedding, kink bands, and convergent fault fans. The frequency and intensity of these features increase dramatically above low points at the base of the sandstone unit. These stratigraphic relations suggest buried interstratal karst, the subsurface dissolution of evaporites bounded by insoluble sediments. We infer that the chaotic unit was formed by subsidence and collapse resulting from the dissolution of an evaporite bed or evaporite-rich unit by groundwater, producing dewatering and synsedimentary deformation structures in the overlying sandstone unit, which infilled surface depressions resulting from collapse. In coeval Moroccan rift basins, facies similar to the Blomidon Formation are associated with halite and gypsum beds. The regional extent of the chaotic unit indicates a marked period of desiccation of a playa lake of the appropriate water chemistry. The sedimentary features described here may be useful for inferring the former existence of evaporites or evaporite-rich units in predominantly elastic terrestrial environments

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Mylroie J. E. , Carew J. L. ,
The exposed carbonates of the Bahamas consist of late Quaternary limestones that were deposited during glacio-eustatic highstands of sea level. Each highstand event produced transgressive-phase, stillstand-phase, and regressive-phase units. Because of slow platform subsidence, Pleistocene carbonates deposited on highstands prior to the last interglacial (oxygen isotope substage 5e, circa 125,000 years ago) are represented solely by eolianites. The Owl's Hole Formation comprises these eolianites, which are generally fossiliferous pelsparites. The deposits of the last interglacial form the Grotto Beach Formation, and contain a complete sequence of subtidal intertidal and eolian carbonates. These deposits are predominantly oolitic. Holocene deposits are represented by the Rice Bay Formation, which consists of intertidal and eolian pelsparites deposited during the transgressive-phase and stillstand-phase of the current sea-level highstand. The three formations are separated from one another by well-developed terra-rossa paleosols or other erosion surfaces that formed predominantly during intervening sea-level lowstands. The karst landforms of San Salvador consist of karren, depressions, caves, and blue holes. Karren are small-scale dissolutional etchings on exposed and soil-covered bedrock that grade downward into the epikarst, the system of tubes and holes that drain the bedrock surface. Depressions are constructional features, such as swales between eolian ridges, but they have been dissolutionally maintained. Pit caves are vertical voids in the vadose zone that link the epikarst to the water table. Flank margin caves are horizontal voids that formed in the distal margin of a past fresh-water lens; whereas banana holes are horizontal voids that developed at the top of a past fresh-water lens, landward of the lens margin. Lake drains are conduits that connect some flooded depressions to the sea. Blue holes are flooded vertical shafts, of polygenetic origin, that may lead into caves systems at depth. The paleokarst of San Salvador is represented by flank margin caves and banana holes formed in a past fresh-water lens elevated by the last interglacial sea-level highstand, and by epikarst buried under paleosols formed during sea-level lowstands. Both carbonate deposition and its subsequent karstification is controlled by glacio-eustatic sea-level position. On San Salvador, the geographic isolation of the island, its small size, and the rapidity of past sea level changes have placed major constraints on the production of the paleokarst

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Cunningham Ki, Northup De, Pollastro Rm, Wright Wg, Larock Ej,
Lechuguilla Cave is a deep, extensive, gypsum- and sulfur-bearing hypogenic cave in Carlsbad Caverns National Park, New Mexico, most of which (> 90%) lies more than 300 m beneath the entrance. Located in the arid Guadalupe Mountains, Lechuguilla's remarkable state of preservation is partially due to the locally continuous Yates Formation siltstone that has effectively diverted most vadose water away from the cave. Allocthonous organic input to the cave is therefore very limited, but bacterial and fungal colonization is relatively extensive: (1) Aspergillus sp. fungi and unidentified bacteria are associated with iron-, manganese-, and sulfur-rich encrustations on calcitic folia near the suspected water table 466 m below the entrance; (2) 92 species of fungi in 19 genera have been identified throughout the cave in oligotrophic (nutrient-poor) ''soils'' and pools; (3) cave-air condensate contains unidentified microbes; (4) indigenous chemoheterotrophic Seliberius and Caulobacter bacteria are known from remote pool sites; and (5) at least four genera of heterotrophic bacteria with population densities near 5 x 10(5) colony-forming units (CFU) per gram are present in ceiling-bound deposits of supposedly abiogenic condensation-corrosion residues. Various lines of evidence suggest that autotrophic bacteria are present in the ceiling-bound residues and could act as primary producers in a unique subterranean microbial food chain. The suspected autotrophic bacteria are probably chemolithoautotrophic (CLA), utilizing trace iron, manganese, or sulfur in the limestone and dolomitic bedrock to mechanically (and possibly biochemically) erode the substrate to produce residual floor deposits. Because other major sources of organic matter have not been detected, we suggest that these CLA bacteria are providing requisite organic matter to the known heterotrophic bacteria and fungi in the residues. The cavewide bacterial and fungal distribution, the large volumes of corrosion residues, and the presence of ancient bacterial filaments in unusual calcite speleothems (biothems) attest to the apparent longevity of microbial occupation in this cave

Combined use of environmental isotopic and hydrochemical data in differentiation of groundwater flow patterns through the Aladağ karstic aquifer-Turkey, Application of Tracers in Arid zone Hydro, 1995,
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Bayari C. S. , Gunay G.
Distinction between the different groundwater flow systems in karstic areas constitutes one of the major objetives of the basin-wide hydrogeologic research. Use of environmental isotopic and hydrochemical investigation techniques provide a great deal of information for the identification of regional groundwater flow systems. The Lower Zamantı Basin, located in the eastern Taurids, presents an accountable water resource potential that can be used for hydroelectric power production. The basin, with the elevation range between 400 m and 350 m, occupies a catchment area of 2000 km2. Humid and semi-arid climatic regimes prevail in the southern and northern parts of the basin. The carbonate rocks and the overlaying impervious ophiolite nappe constitute the major geologic units in the area. Systematic hydrochemical and environmental isotopic surveys have been carried out to discriminate between the different groundwater flow systems existing in the basin. Hydrochemical studies have been conducted by insitu measurements, sampling and analyses of water samples from about 80 points. Based on the results of hydrochemical evaluations, 23 sampling points, including streams and karstic springs, have been selected for environmental isotopic survey. The integrated evaluation of the available data indicates clearly that two different groundwater flow patterns exist in the basin; namely a shallow flow and a deep regional flow. The characteristic values of temperature, electrical conductivity, carbonate alkalinity and log PCO2 of the shallow-flow in the karstic effluents fed by shallow groundwater circulation springs are 8C, 80 S/cm, 1.5 meq/l and 10-2 atm, respectively. On the other hand, higher values, such as 15C, 455 S/cm, 5.0 meq/l and 10-1 atm are observed in the springs fed by deep-regional groundwater flow. The tritium data indicate that the springs fed by the deep-regional groundwater have longer residence times. Moreover, the recharge area elevations, as envisaged from the oxygen-18 data, also provide supporting evidence for the distinction of different groundwater flow patterns. Additionally, comparison of groundwater temperature with oxygen-18 content presents reliable information to understand the possible interaction among the different karstic effluents.

Sannur Cave: A Crescent shaped cave developed in Alabaster formation in Eastern Desert, Egypt, 1995,
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Gü, Nay G. , Elbedewy F. , Ekmekci M. , Bayari S. , Kurttas T.
An expedition to Egypt set out to explore the Wadi Sannur where no speleological work had taken place. The most notable karst feature identified to date is the Sannur Cave, the largest subterranean chamber known in Egypt. It is situated about 70 km to the southeast of Beni-Suef city in the remote Wadi Sannur of the Eastern Desert where the main rock units belong to Eocene and Pliocene periods. The Eocene is represented by limestone including alabaster which is known to be quarried first by the ancient Egyptians. Sannur Cave is first explored during blasting in the alabaster quarry which caused an artificial entrance to the cave. The cave is a single crescent shape chamber approximately 275 m long and can be arbitrarily divided into two sectionshaving different characteristics; left side gallery and right side gallery. Few speleothems occur in the left side gallery while the right side gallery is decorated intensively with many kinds of spelethem including stalagtites, stalagmites, flowstones, microgours, helictites and soda-straws etc. In addition to surveying the cave, based on the geologic, structural and morphologic observation inside and outside the cave some interpretations on the paleoenvironment an the origin of the cave. Surveying was performed with grade 5D according to BCRA Gradings.

Breakdown development in cover beds, and landscape features induced by intrastratal gypsum karst., 1996,
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Andrejchuk Vjacheslav, Klimchouk Alexander
Intrastratal karst is by far the predominant gypsum karst type. Its development may begin in deep-seated settings within rocks already buried by younger strata, and it proceeds increasingly rapidly as uplift brings gypsum sequences into progressively shallower positions. Such development commonly occurs under confined (artesian) hydrogeological conditions, that subsequently change to open conditions (phreatic-water table-vadose). The general evolutionary line of intrastratal karst is typified by progressive emergence of a sequence into a shallower position, activation of groundwater circulation and development of cave systems within karst units, commencement of gravitational breakdown and its upward propagation through overlying beds, and development of a karst landscape. These processes and phenomena progress through the directed evolution of karst types as follows: deep-seated intrastratal karst (1K) to subjacent 1K to entrenched 1K to denuded karst. One of the main characteristics of intrastratal karst is that it induces gravitational breakdown in cover beds. With the aid of processes other then simple breakdown, such effects may propagate upwards and may, or may not, reach the surface, depending upon the thickness and structure of the overburden. A karst landscape evolves when such features reach the surface. This paper considers the conditions and mechanisms of such development.

The gypsum karst of Italy., 1996,
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Forti Paolo, Sauro Ugo
Gypsum karst has been studied in Italy since the last decades of the l9th Century. In 1917 the geographer Olinto Marinelli published "Fenomeni carsici delle regioni gessose d'Italia", a fundamental synthesis of the early research. He distinguished 56 different morpho-karstic gypsum units and/or areas, which are all different in size and character, and described them, paying special attention to their surface morphology and hydrology. Marinelli listed all the main gypsum units and only a few secondary outcrops were overlooked. After Marinelli's synthesis, except for some discussion of archaeological caves, only a few papers about gypsum karst and environment were published until the nineteen-fifties. In the nineteen-sixties and seventies much exploratory work and documentation was carried out in the Emilia Romagna area, principally devoted to the gypsum caves, and undertaken by the local speleological clubs and university researchers. The chapters that describe gypsum karst surface landforms in this publication contain many references to examples of gypsum karst in Italy, and these supplement the descriptions provided below.

Gypsum karst of the Baltic republics., 1996,
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Narbutas Vytautas, Paukstys Bernardas
The Baltic Republics of Estonia, Latvia and Lithuania have karst areas developed in both carbonate and gypsiferous rocks. In the north, within the Republic of Estonia, Ordovician and Silurian limestones and dolomites crop out, or are covered by glacial Quaternary sediments. To the south, in Latvia and Lithuania, gypsum karst is actively developing in evaporites of Late Devonian (Frasnian) age. Although gypsum and mixed sulphate-carbonate karst only occupy small areas in the Baltic countries, they have important engineering and geo-ecological consequences. Due to the rapid dissolution of gypsum, the evolution of gypsum karst causes not only geological hazards such as subsidence, but it also has a highly adverse effect on groundwater quality. The karst territory of the Baltic states lies along the western side of the area, called the Great Devonian Field that form part of the Russian Plain. Within southern Latvia and northern Lithuania there is an area, exceeding 1000 sq. km, where mature gypsum karst occurs at the land surface and in the subsurface. This karst area is referred to here as the Gypsum Karst Region of the Baltic States. Here the surface karst forms include sinkholes, karst shafts, land subsidence, lakes and dolines. In Lithuania the maximum density of sinkholes is 200 per sq. km; in Latvia they reach 138 units per sq. km. Caves, enlarged dissolution voids and cavities are uncommon in both areas.

The combined use of Sr-87/Sr-86 and carbon and water isotopes to study the hydrochemical interaction between groundwater and lakewater in mantled karst, 1996,
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Katz B. G. , Bullen T. D. ,
The hydrochemical interaction between groundwater and lakewater influences the composition of water that percolates downward from the surficial aquifer system through the underlying intermediate confining unit and recharges the Upper Floridan aquifer along highlands in Florida. The Sr-87/Sr-86 ratio along with the stable isotopes, D, O-18, and C-13 were used as tracers to study the interaction between groundwater, lakewater, and aquifer minerals near Lake Barco, a seepage lake in the mantled karst terrane of northern Florida. Upgradient from the lake, the Sr-87/Sr-86 ratio of groundwater decreases with depth (mean values of 0.71004, 0.70890, and 0.70852 for water from the surficial aquifer system, intermediate confining unit, and Upper Floridan aquifer, respectively), resulting from the interaction of dilute oxygenated recharge water with aquifer minerals that are less radiogenic with depth. The concentrations of Sr2 generally increase with depth, and higher concentrations of Sr2 in water from the Upper Floridan aquifer (20-35 mu g/L), relative to water from the surficial aquifer system and the intermediate confining unit, result from the dissolution of Sr-bearing calcite and dolomite in the Eocene limestone. Dissolution of calcite [delta(13)C = -1.6 permil (parts per thousand)] is also indicated by an enriched delta(13)C(DIC) (-8.8 to -11.4 parts per thousand) in water from the Upper Floridan aquifer, relative to the overlying hydrogeologic units (delta(13)C(DIC) < -16 parts per thousand). Groundwater downgradient from Lake Barco was enriched in O-18 and D relative to groundwater upgradient from the lake, indicating mixing of lakewater leakage and groundwater. Downgradient from the lake, the Sr-87/Sr-86 ratio of groundwater and aquifer material become less radiogenic and the Sr2 concentrations generally increase with depth. However, Sr2 concentrations are substantially less than in upgradient groundwaters at similar depths. The lower Sr2 concentrations result from the influence of anoxic lakewater leakage on the mobility of Sr2 from clays. Based on results from mass-balance modeling, it is probable that cation exchange plays the dominant role in controlling the Sr-87/Sr-86 ratio of groundwater, both upgradient and downgradient from Lake Barco. Even though groundwater from the three distinct hydrogeologic units displays considerable variability in Sr concentration and isotopic composition, the dominant processes associated with the mixing of lakewater leakage with groundwater, as well as the effects of mineral-water interaction, can be ascertained by integrating the use of stable and radiogenic isotopic measurements of groundwater, lakewater, and aquifer minerals

Agricultural chemicals at the outlet of a shallow carbonate aquifer, 1996,
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Felton Gk,
A groundwater catchment, located in Woodford and Jessamine Counties in the Inner Bluegrass of Kentucky, was instrumented to develop long-term flow and water quality data. The land uses on this 1 620-ha catchment consist of approximately 59% in grasses consisting of beef farms, horse farms, and a golf course; 16% row crops; 6% orchard; 13% forest; and 6% residential. Water samples were analyzed twice a week for, Ca, Mg, Na, Cl-, HCO3-, SO4=, NO3-, total solids, suspended solids, fecal coliforms, fecal streptococci, and triazines. Flow rate and average ambient temperature were also recorded. No strong linear relationship was developed between chemical concentrations and other parameters. The transient nature of the system was emphasized by one event that drastically deviated from others. Pesticide data were summarized and the ''flushing'' phenomena accredited to karst systems was discussed. The total solids content in the spring was consistent at approximately 2.06 mg/L. Fecal bacteria contamination was well above drinking water limits (fecal coliform and fecal streptococci averages were 1 700 and 4 300 colony-forming-units/100 mL, respectively) and the temporal variation in bacterial contamination was not linked to any other variable

Hydrogeological study and discharge features of the Niksar karst springs (Tokat-Turkey), 1997,
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Syed M. A. , Afsin M. , Celik M. ,
The exposed Paleozoic and Recent units in the study area have various hydrogeological characteristics such as pervious, semipervious, and impervious. Pervious limestones and associated impervious formations that were not influenced by tectonic movement are connected to produce karst springs. This paper presents the relationship between the discharge coefficient and other aquifer properties by using the hydrograph analyses of the karst springs. The magnitude of the discharge of the spring apparently controls the character of flow (such as laminar) and conduit in the aquifer. The correlation analysis shows a positive relation between Q(0)-Q(t), Q(0)-storage capacity, Q(t)-storage capacity, and alpha-discharge change, These results enhances the properties of the karst springs. Both monthly and annual rainfall contribute to spring discharge. All karst springwaters are suitable for household and food industry uses

Stratigraphy, sedimentology, and structural geology of gypsum caves in east central New Mexico, 1997,
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Forbes J, Nance R,
Hundreds of solution caves have developed in evaporites and carbonates of the Permian San Andres Formation where it crops out between Vaughn and Roswell, New Mexico, USA. Several of the caves are over 3.2 km (2 miles) in length, and the deepest has a vertical extent of over 120 m (400 feet). These gypsum caves afford an extraordinary opportunity to examine the evaporite rocks in which they are developed. We have examined interbedded gypsum and dolostone strata exposed in the walls of 11 of these caves, and show stratigraphic sections on two geologic cross sections. Gypsum textures exposed in the caves include massive, nodular, and laminar types. While we refer to them as ''gypsum caves,'' gypsum is not the only lithology exposed. Some cave passages and rooms are developed in thick dolostone units intercalated with or overlain by gypsum beds. Correlation of beds exposed in two or more caves has allowed us to infer the local geologic structure. The sedimentary sequence penetrated by a cave exerts a profound effect on the geometry and passage cross-section of the cave. Many cave passages have gypsum walls and a dolostone or limestone floor. Although many of the cave passages flood completely during major storm events, the stairstep profile of most of the caves is indicative of speleogenesis that has occurred predominantly within the vadose zone

Richard Lake, an evaporite-karst depression in the Holbrook basin, Arizona, 1997,
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Neal J. T. , Colpitts R. M. ,
Richard Lake is a circular depression 35 km SE of Winslow, Arizona, about 1.6 km wide and with topographic closure of 15-23 m. The depression is 5 km south of McCauley Sinks, another depressed area about 2 km wide which contains some 40 large sinkholes. Richard Lake formerly contained water after heavy rains prior to headwater drainage modification but is now dry most of the time. It is situated within the Moenkopi / Kaibab outcrop belt with Coconino Sandstone at shallow depth near the southwestern margin of the subsurface Permian evaporite deposit in the Holbrook Basin. Outcropping strata are predominantly limestone, but the salt-karst features result from collapse of these units into salt-dissolution cavities developed in the Corduroy Member of the Schnebly Hill Formation of the Sedona Group (formerly called the Supai Salt) that underlies the Coconino. Richard Lake is interpreted as a collapse depression containing concentric faults, pressure ridges, and a 200m wide sinkhole in the center. A second set of pressure ridges parallels the axis of the nearby western end of the Holbrook Anticline, trending generally N 30 degrees W. In the alluvium at the bottom of the central sinkhole, two secondary piping drain holes were observed in early 1996. Northwest-trending fissures also were observed on the depression flanks, essentially parallel to the regional structure. The presence of Richard Lake amidst the preponderance of salt-karst features along the Holbrook Anticline suggests a similar origin by salt dissolution, but with distinct manifestation resulting from variation in overburden thick?less and consolidation. Similarities of origin between Richard Lake and McCauley Sinks seem likely, because of their similar geological setting, size, appearance, and proximity. Two lesser developed depressions of smaller dimensions occur in tandem immediately west along a N 62 degrees W azimuth. Secondary sinkholes occur within each of these depressions, as at Richard Lake. Breccia pipes are apt to be found beneath all of these structures

Turbidity and microorganisms in a karst spring, 1997,
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Nebbache S. , Loquet M. , Vinceslasakpa M. , Feeny V. ,
This study was focused mainly on relations between turbidity and bacterial contamination of a karst spring. The data from the resurgence site show that for a turbidity <1,2 NTU, the spring is benefits of good sanitary conditions. The highest degree of bacterial contamination generally coincides with increased rainfall (automn and winter). This turbidity is also a factor of enhancing survival in particular-for fecal bacteria. correlations are established between turbidity and fecal bacteria, Those data show different origins of suspended particulate matters. The latter are transfered with superficial waters and rapid throughflow, or with water stored in 'systemes annexes karstifies' (storage units) then flushed out. Following the study of the first peak of turbidity, after recession, we find that turbidity is essentially due to the P3 class of particles (4.3 to 11 mu m) and that some microorganisms are carried by the following classes of articles: ammonifiers by class P1 (<1.7 mu m), mesophilic microflora by P2 (1.7 to 4.3 mu m), fecal streptbcocci by P3 (4.3 to 11 mu m), fecal coliforms and denitrifiers by P4 (11 to 27 mu m). A knowledge of turbidity and bacterial contamination relationships suppose to take into account the stational ecological events and the hydrodynamic of the karst but also the adhesion laws between bacteria and particles

Microorganisms as tracers in groundwater injection and recovery experiments: a review, 1997,
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Harvey R. W. ,
Modern day injection and recovery techniques designed to examine the transport behavior of microorganisms in groundwater have evolved from experiments conducted in the late 1800s, in which bacteria that form red or yellow pigments were used to trace flow paths through karst and fractured-rock aquifers. A number of subsequent groundwater hydrology studies employed bacteriophage that can be injected into aquifers at very high concentrations (e.g., 10(13) phage ml(-1)) and monitored through many log units of dilution to follow groundwater flow paths for great distances, particularly in karst terrain. Starting in the 1930s, microbial indicators of fecal contamination (particularly coliform bacteria and their coliphages) were employed as tracers to determine potential migration of pathogens in groundwater. Several injection and recovery experiments performed in the 1990s employed indigenous groundwater microorganisms (both cultured and uncultured) that are better able to survive under in situ conditions. Better methods for labeling native bacteria (e.g. by stable isotope labeling or inserting genetic markers, such as the ability to cause ice nucleation) are being developed that will not compromise the organisms' viability during the experimental time course

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